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During the 22 months of a collaborative study carried out in Almirante, Panama, 1,341 pools comprising 181,979 mosquitoes and Phlebotomus sandflies were inoculated into suckling mice and hamster kidney cell cultures. Thirty-nine viruses were thus isolated which were classified as 9 types of 5 recognized groups, and one previously undescribed, totally distinct agent. Previously unknown on the Isthmus of Panama were: Una virus of group A, Guaroa, Cache Valley and Wyeomyia-complex viruses of the Bunyamwera group, and a member of the Guama group. The most noteworthy finding was the repeated isolation from Phlebotomus sandflies of Indiana type vesicular stomatitis virus and of the apparently new agent.
* Present address: Laboratory of Virology and Rickettsiology, DBS, Bldg. 29, Rm. 316, National Institutes of Health, Bethesda, Md. 20014.
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