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Thin-Layer and Gas-Liquid Chromatographic Analysis of Lipid from Cercariae of Schistosoma Mansoni^*

Thomas M. Smith, Thomas J. Brooks, Jr. AND Harold B. White, Jr.^,,

The lipid class composition of cercariae of Schistosoma mansoni has been examined by thinlayer chromatography. Phospholipid, free sterol, and sterol ester were major components of the mixture. Triglyceride, partial glycerides, and free fatty acids were also present.

A preliminary examination of the total higher fatty acids of cercariae of S. mansoni revealed that a complex mixture was present containing a minimum of 25 components. Palmitic, stearic, and oleic acids were major components. At least 20% of the mixture was composed of fatty acids containing 20 carbons or more.

^* This investigation was supported in part by USPHS grant AM 06895, National Institute of Arthritis and Metabolic Diseases.

Department of Biochemistry, University of Mississippi Medical Center, 2500 North State Street, Jackson, Mississippi 39216.

Department of Preventive Medicine, University of Mississippi Medical Center, 2500 North State Street, Jackson, Mississippi 39216.

Research Career Development Awardee (6-K3-HE-18,345), United States Public Health Service.