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A Study of the Occurrence of Multi-Nucleate Trophozoites of Three Strains of Entamoeba histolytica in the Shaffer-Frye and CLG Media^*

The occurrence of trophozoites in cultures of three strains of Entamoeba histolytica with two or more nuclei has been investigated. It was found that in either the Shaffer-Frye or CLG medium, strain K-9 had 10 to 13 percent of 2-nucleate trophozoites beginning somewhere between 8 and 16 hours and continuing through 120 hours of incubation. With strain 103, the percentage varied from 2 to 6 percent and with strain 200 it was 3 to 25 percent. No bi-nucleate amebae were present in any cultures at 4 or 8 hours of incubation. A few multinucleate trophozoites (3 to 5 nuclei) were seen in cultures of strains K-9 and 200. These apparently occurred without relation to the growth cycle of the culture. With strain 103 only one multinucleate trophozoite was seen in several experiments. It is suggested that these observations indicate that nuclear division and cell division may be two separate phenomena that do not necessarily run parallel.

^* The observations reported here were supported by a research grant (AI-03655-03) from the National Institute of Allergy and Infectious Diseases, United States Public Health Service.