AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 13(5), 1964, pp. 667-673
Copyright © 1964 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nelson, E. C.
Right arrow Articles by Jones, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nelson, E. C.
Right arrow Articles by Jones, M. M.

Cultivation of Entamoeba Histolytica in Carbon Dioxide-Bicarbonate Buffer System Media*

E. Clifford Nelson AND Muriel M. Jones
Department of Microbiology, Medical College of Virginia, Richmond, Virginia

Procedures for the cultivation of Entamoeba histolytica in CO2-bicarbonate buffer system media are described. A simple medium composed of Hanks BSS, 1.4 percent sodium bicarbonate solution, horse serum and rice powder has proven reliable for the isolation and continued maintenance of strains accompanied by a mixed bacterial flora. Strains have been maintained for periods up to 4 years. Growth and encystment follow a pattern apparently corresponding to that in the intestinal environment. The prompt and vigorous growth response of strains indicates that the system meets the physiological requirements and may even afford a considerable degree of simulation of the in vivo environment. A direct microscopic method of studying cultures has facilitated study of strains under cultivation. Preliminary observations show that basic characteristics are not altered by prolonged cultivation. Strain differences in regard to growth, encystment, migration, response to pH and temperature have been noted. Application to the differentiation of strains is suggested.


* This investigation was supported by Public Health Service grant AI-03826 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Public Health Service. Presented in part at the Annual meeting of the American Society of Tropical Medicine and Hygiene, Washington, D. C., 1961.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1964 by the American Society of Tropical Medicine and Hygiene.