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Studies on the Ameba-Bacteria Relationship in Amebiasis

III. Induced Amebic Lesions in the Germfree Guinea Pig

An inoculum of Entamoeba histolytica, prepared by regular procedures, that was shown incapable of producing lesions in germfree guinea pigs in our previously reported studies gave the same results in the present investigations. However, an inoculum of the ameba cultivated and harvested by modified procedures, which reduced handling and exposure to oxygen and seemed to enhance the vigor of the ameba, produced amebic lesions in the germfree guinea pig. Most of the lesions appeared to originate at the site of the puncture wound through the cecal wall. When the inoculum was introduced without trauma to the cecal wall the number of cecal lesions was significantly reduced. E. histolytica scraped from amebic lesions in germfree guinea pigs and introduced into other germfree guinea pigs produced lesions in a greater percentage of animals than in vitro grown amebas. The amebic infections in germfree animals were purely tissue infections with no evidence of propagation of the ameba in the lumen of the germfree intestine. Fatal amebic enteritis occurred frequently in conventional guinea pigs inoculated as controls for the germfree experiments. There were no fatalities from a similar cause among inoculated germfree animals observed for the same post-inoculation periods.