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Studies on Trachoma

II. Comparison of Fluorescent Antibody, Giemsa, and Egg Isolation Methods for Detection of Trachoma Virus in Human Conjunctival Scrapings^*

Roger L. Nichols, Dorothy E. McComb, Nadim Haddad AND Edward S. Murray

A controlled study of three methods—fluorescent antibody, Giemsa, and egg inoculation—for the detection of trachoma virus in human conjunctival scrapings is presented. The morphology of trachoma inclusions as shown by specific immunofluorescence is described. Evidence for the superiority of the fluorescent antibody method to Giemsa is discussed. Field and laboratory applications of this method are suggested.

^* This study was supported in part by the Arabian American Oil Company under its agreement with Harvard University for the conduct of the Aramco Trachoma Research Program.

With the technical assistance of Miss Clare Conover.