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Studies on the Growth Requirements of Entamoeba Histolytica

VIII. Thermolability of the Growth-Promoting Properties of the CLG and Shaffer-Frye Media^*

The heat lability of the growth promoting ability of the CLG and S-F media for the propagation of Entamoeba histolytica has been studied.

The supernatant fluids used in these media were subjected to heat in a water bath. The critical temperature on heating for 15 minutes for the CLG medium appears to be 55°C and for the S-F medium 53°C.

Enhanced propagation of E. histolytica was observed after 24 hours and sometimes after 48 hours in these heated supernatant fluids. It is suggested that this results either from the release of a stimulatory substance or the destruction of an inhibitory one by heat. Evidence indicates that this is different from the heat labile factor necessary for continued propagation.

^* Aided by a research grant from the National Institutes of Allergy and Infectious Diseases, National Institutes of Health, U. S. Public Health Service.