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Am. J. Trop. Med. Hyg., 12(1), 1963, pp. 103-116
Copyright © 1963 by The American Society of Tropical Medicine and Hygiene

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Precise Standardization of Reagents for Complement Fixation

John F. Kent AND Earl H. Fife, Jr.
Division of Laboratories and Research, New York State Department of Health, Albany, and Department of Serology, Division of Communicable Disease and Immunology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C.

A precise complement-fixation test, applied diversely in the serodiagnosis of infectious disease, is described. Detailed quantitative methods for standardization and control of the hemolytic system are given. Complement fixation is shown to be affected by the concentration of nonantibody serum components, as well as by the conditions (time and temperature) of primary incubation, and the influence of these factors on antigen standardization is illustrated with examples from the reactions of treponemal, trypanosomal, and trichinal antigens with homologous antisera.




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