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Am. J. Trop. Med. Hyg., 11(6), 1962, pp. 790-795
Copyright © 1962 by The American Society of Tropical Medicine and Hygiene

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Isolation of Histoplasma Capsulatum and Microsporum Gypseum from Soil and Bat Guano in Panama and the Canal Zone

Robert L. Taylor*, Martha H. Shacklette AND Harlan B. Kelley
Mycology Section, Middle America Research Unit, Balboa Heights, Canal Zone

Eight potential areas of histoplasmosis endemicity in the Republic of Panama and the Canal Zone were located by isolation of Histoplasma capsulatum from soil or bat guano. Endemic sites include a flower garden, a bat cave, a tree frequented by bats and a previously unrecognized ecologic environment associated with tree buttresses in tropical forests.

The microorganism was shown to persist in the soil at known positive sites by repeated recovery at the beginning, end and during both the rainy and dry seasons.

H. capsulatum was recovered from livers and spleens of fructiverous bats 11 weeks after intraperitoneal inoculation with the mycelial phase of the microorganism.

Microsporum gypseum was recovered from 31% of the specimens processed using a human hair bait culture technique.


* Major, Medical Service Corps, United States Army. Present address: Department of Bacteriology, Walter Reed Army Institute of Research, Washington 12, D. C.







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Copyright © 1962 by the American Society of Tropical Medicine and Hygiene.