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Antigenic Analysis of Entamoeba Histolytica by means of Fluorescent Antibody

III. Reactions of the Laredo Strain with Five Anti-Histolytica Sera

A strain of Entamaeba histolytica-like ameba (Laredo) cultured at both 37° and 25°C, E. moshkovskii also growing at both temperatures, and E. histolytica K9 were exposed to five anti-histolytica sera conjugated with fluorescein, as well as to a normal serum control.

Laredo at 37°C stained similarly to E. histolytica K9 with four of the antisera, whereas E. moshkovskii at the same temperature stained to a distinctly lesser degree. With the fifth antiserum, Laredo at 37°C stained much less than E. histolytica K9, whereas E. moshkovskii did not stain significantly at all. Laredo at 25°C stained distinctly less than E. histolytica K9 and was similar to E. moshkovskii at both temperatures in this respect.

It is concluded that the Laredo strain resembles E. histolytica antigenically more than E. moshkovskii does, but is not completely typical of E. histolytica in its staining reactions with fluorescent antisera.

^* Present address: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.