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Am. J. Trop. Med. Hyg., 11(1), 1962, pp. 141-148
Copyright © 1962 by The American Society of Tropical Medicine and Hygiene

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Encephalitis on Taiwan*

III. Virus Isolations from Mosquitoes

San-Pin Wang, J. Thomas Grayston{dagger} AND Stephen M. K. Hu{ddagger}
United States Naval Medical Research Unit No. 2, Taipei, Taiwan

Over 100,000 Culex tritaeniorhynchus and over 150,000 Culex fuscocephalus mosquitoes caught on Taiwan throughout the year were tested for virus by inoculation into infant mouse brain. Virus was isolated from 22 pools of the former and 1 pool of the latter species. No virus was isolated from 9 other species tested. All but one virus isolation came from mosquitoes collected between July 3 and 26, 1958, and June 3 and 26, 1959. All positive C. tritaeniorhynchus pools were collected in northern Taiwan. The one positive C. fuscocephalus pool came from southern Taiwan. All isolated virus strains were shown to belong to the Japanese encephalitis virus group by complement fixation test. Neutralization tests with 6 Taiwan virus strains showed close similarity among the strains and prototype JE virus. It is concluded that C. tritaeniorhynchus is the important vector of JE virus in northern Taiwan.


* This study was supported in part by contract Nonr-2121(07) between the Office of Naval Research and the University of Chicago, and in part by funding under Public Law 480, Section 104(c). The opinions and assertions contained herein are those of the authors and are not to be construed as official or reflecting the views of the Navy Department or the Naval Service at large.


{dagger} Present address: Department of Preventive Medicine, University of Washington, Seattle 5, Washington.


{ddagger} Present address: United States Operation Mission, Nepal.






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Copyright © 1962 by the American Society of Tropical Medicine and Hygiene.