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SUPPLEMENTAL FIGURE 1. Anti-penta-His Western blot of recombinant Plasmodium vivax reticulocyte binding protein 1 (PvRBP1) and P. vivax Duffy binding protein region II (PvDBP-RII) proteins. Approximately 2 µg of each protein were run on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane. Western blot was performed using an anti-penta-His monoclonal antibody (Qiagen, Valencia, CA) according to the manufacturer’s specifications. See "Materials and Methods" for details on expression and purification of proteins. Molecular weights in kilodaltons (kDa) are indicated.